Fetal Bovine sera

Manufacturer: J R Scientific

US Origin Fetal Bovine Serum (FBS) is the most widely used FBS in the world. Although US Origin FBS is considered the world’s standard for quality it is never the less becoming a lower standard in the view of the OIE (World Organization for Animal Health) and other regulatory bodies. On a scale of 1-4 for geographical BSE risk (GBR), with 1 being the lowest risk and 4 being the highest risk, US FBS ranks a 3 – demonstrating a moderate to high risk that BSE exists in the USA. US Origin FBS provided by J R Scientific is of the highest quality available in the USA. It is collected using state of the art aseptic collection techniques resulting in low endotoxin and hemoglobin concentrations. 

For a full serum product listing, click here!

Fast Tissue-to-PCR Kit (#K1091) - Rapid extraction and amplification of DNA

Manufacturer: Fermentas

The Fast Tissue-to-PCR kit is a complete system for rapid extraction and amplification of genomic DNA from animal tissues.There is no need for procedures as tissue mechanical disruption, overnight digestion, phenol extraction, DNA precipitation or column purification.

The procedure involves short 10 minute incubation of the sample in the Tissue Lysis Solution supplemented with Proteinase K at room temperature. Then the Neutralization Solution T is added, and the tissue extract is ready for PCR with the Tissue Green PCR Master Mix (2X).

Feature / Benefits: 

• Fast sample lysis during 10 min incubation at room temperature
• Efficient PCR genotyping
• Direct loading of PCR product on gel
• Compatibility with various sample material: tissues from mice, fish and insects, human hair and saliva

Applications

• Genotyping
• Transgene detection
• Knockout analysis
• Sequencing

Components

• Tissue Lysis Solution
• Proteinase K
• Neutralization Solution T
• Tissue Green PCR Master Mix (2X)
• Water, nuclease-free
• Detailed Protocol

Figure 2. Multiplex PCR. 
Genomic DNA from mouse tail, ear clip and finger was extracted and amplified with Fast Tissue-to-PCR Kit. 114, 214 and 388 bp PCR products were generated using three sets of primer pairs.
M – GeneRuler™ Express DNA Ladder (#SM1551)
1, 2 – mouse ear clip
3, 4 – mouse tail
5, 6 – mouse finger

Epitope Tag Antibody – Detection of Tag-fusion proteins

Manufacturer: Nacalai Tesque

Nacalai Tesque carries an impressive variety of antibodies.Apart from a wide range of secondary antibodies, recently tag antibodies are developed in increased numbers. Currently available tagged antibody types are: GFP, c-Myc, His, V5, GST. Most of these types are available in POD coupled form as well. Uniquely, GFP (Rat IgG2a) is suitable for immunohistochemical detection!

Feature / Benefits: 

• Immunohistochemical application
• Rat monoclonal antibody

Specifications:

Signal Enhancer Hikari - Signal Enhancer for Western Blotting and ELISA

Manufacturer: Nacalai Tesque

Signal Enhancer HIKARI resolve the problems of low sensitivity and high background often encountered during procedures such as Western blotting and ELISA. Witness a more effective assay with Signal HIKARI instead of conventional diluents such as PBS and TBS to detect the protein of interest.

Features:

• Enhances antigen-antibody reactions 
• Reduces background 
• Works with any substrate 
• Ready-to-use reagent 

Ordering information

 

 

Detection enhancement of Rac1 with treatment of HIKARI in Western blotting

Treatment with Signal Enhancer HIKARI resulted in clear signal, whereas dilution with conventional PBS-T resulted in no signal.

Signal Enhancer Hikari for Immunostain – Detect protein of interest with Immunostain procedures

Manufacturer: Nacalai Tesque

Signal Enhancer HIKARI for Immunostain resolves the problems of low sensitivity and high background often encountered during immunostain procedures. Signal Enhancer HIKARI produces a significant increase in the ability to detect protein of interest and eliminate unwanted background.

Feature / Benefits:

• Enhances signals 
• Reduces background
• Ready-to-use reagent
• Works with any system - applicable to colorimetric, chemiluminescent and fluorescent detection

Specifications: Product information

*Note: Signal Enhancer HIKARI for Immunostain Solution A and B exhibit different enhancing effects, depending on antigens and antibodies. These solutions can be used independently and a Trial set is available to help researchers select the suitable solution for their use.

Brain tissue section was stained with secondary antibody conjugated with AF555 (red) and counter stained with Hoechst dye (blue).

RNAGEM – Extraction of RNA from mammalian cell culture

Manufacturer: ZyGEM

RNAGem™ tissue RNA extraction kits feature a new formulation of enzymatic technologies that uses a single-tube process to provide complete extraction of RNA. This includes smaller RNA molecules such as noncoding RNA and microRNA that may be excluded by other RNA extraction methods. The RNAGem kits deliver high molecular weight RNA and good transcriptome profiles. They are suitable for complete gene-expression and noncoding RNA analyses.

Feature / Benefits:

• Takes less than 10 minutes from start to finish, including handling time
• Requires a single 5 minute incubation
• Does not use GITC, phenol, chloroform or isopropanol
• Delivers linear yields of total Nucleic Acid from 1 to 105 cells
• Delivers linearity that is matched between high and low copy genes
• Does not discriminate RNA molecules based on size

Kit Components of RNAGEM™ Tissue

• RNAGEM enzyme
• 10 x SILVER buffer
• 10 x TE storage buffer
• QuickStart Guide

Aviva Western Blot Reagent Kit

Manufacturer: Aviva Systems Biology

Aviva Systems Biology's new Western Blot Reagent Kits provide all the reagents required for conducting high quality, validated western blot analysis in one simple kit. Conditions and protocols have been optimized for easy visualization of each specific protein target.

Each kit includes: 

Primary Target Specific Antibody - WB Validated
Positive Control Lysate - Insures Antibody is Performing Properly 
Blocking Peptide - To Confirm Antibody Target Specificity
Secondary Antibody - Suitable for WB Analysis

Features/Benefits:

• Includes all reagents required for running validated Western Blotting assays
• Simple and validated protocols 
• 84 kits available in a comprehensive range of key target areas

For more information please click here.

DNAgard™ Tissues & Cells - Stabilize DNA in tissue for at least 2 months at ambient temperature

Manufacturer: Biomatrica

DNAgard is a new Biomatrica product for the preservation and storage of DNA in tissues and cell culture lines at room temperature. DNAgard is an aqueous, non-toxic tissue and cell storage solution that permeates cells and stabilizes intracellular DNA in unfrozen samples. A unique dry-down feature allows extended storage time for one year at room temperature. Extraction by standard techniques yields high quality DNA ready for all downstream analyses.

Find out more on DNAgard™ Tissues & Cells and other products from Biomatrica here.

Universal Reference Products

Manufacturer: Biochain

Universal Total RNA, cDNA and Total Protein

• From Human, Dog, Monkey, Mouse, Rat, and Chicken
• Male and Female Adults from Each Species
• Broad Representation of Genes and Proteins

Features/Benefits:

• Made from very diversified tissues for broad gene / protein coverage
• Industrial production scale to minimize lot-to-lot variation
• High quality without contamination

Search online to view BioChain’s comprehensive range of Universal Reference Products.

Search for “Universal ” @ http://www.biochain.com/

Restriction Enzymes

Manufacturer: Fermentas

FERMENTAS recently launched their new line of restriction enzymes called FastDigest®.

Fermentas FastDigest® enzymes is a premium line of restriction enzymes for easy digestion of DNA. It save time - Digest DNA in just 5 minutes!

All FastDigest® enzymes work in the same reaction buffer at the same temperature, therefore multiple digestions on DNA in any combination of FastDigest® enzymes are simple!

For more information, please call us.

Features/Benefits:

• All enzymes work in same reaction conditions
• No more sequential digestions
• No more tedious buffer changes
• No more concerns about star activity